Genetic variability of Escherichia coli 0157:H7.
| dc.advisor | Scott Crupper | en_US |
| dc.college | las | en_US |
| dc.contributor.author | Tseng, Ching Wen. | |
| dc.date.accessioned | 2012-06-05T15:02:31Z | |
| dc.date.available | 2012-06-05T15:02:31Z | |
| dc.date.created | 1999 | en_US |
| dc.date.issued | 2012-06-05 | |
| dc.department | biological sciences | en_US |
| dc.description | ix, 61 leaves | en_US |
| dc.description.abstract | Escherichia coli 0157:H7 is recognized as an important food-borne pathogen, being implicated in over 2,500 deaths worldwide. Various genetic typing methods have been investigated in studying possible outbreak strains, including pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPO), and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) . These genetic methods yield information on the genetic diversity of organisms, but make no attempt to identify specific virulence genes. In this study, we present a modified RAPO which targets specific virulence genes under low stringency conditions and also yields information on the diversity of the organism in question. Plasmid profiling, ERIC-I peR, and RAPO were also used to determine genetic heterogeneity for comparison. Our results indicate the modified RAPO may be a better tool for investigating the genetic relationships of organisms. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1205 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Escherichia coli. | en_US |
| dc.title | Genetic variability of Escherichia coli 0157:H7. | en_US |
| dc.type | Thesis | en_US |