Detection of Antibiotic Resistance in the Environment using Functional Metagenomics
| dc.advisor | Scott Crupper | en_US |
| dc.college | las | en_US |
| dc.contributor.author | Park, Younshim | |
| dc.date.accessioned | 2014-07-10T13:42:19Z | |
| dc.date.available | 2014-07-10T13:42:19Z | |
| dc.date.created | May 15, 2014 | en_US |
| dc.date.issued | 2014-07-10 | |
| dc.department | biological sciences | en_US |
| dc.description.abstract | Metagenomicshas the potential to discover novel phenotypes that are not recognizable by DNA sequence analysis. In this study,functional metagenomic analysis of Niangua River soil detected spectinomycin (NR-YP1) and nalidixic acid (NR-YP3) resistance expressed in E. coli EPI300. Subclonesof eachwere generated and screened on spectinomycin ornalidixic acid containingmedia for NR-YP1and NR-YP3, respectively. No subclones were identified conferring resistance to either of the antibiotics. Random DNA sequencing and BLAST analysis of both NR-YP1 and NR-YP3 subclones matchedan environmental clone, termed zdt-9n2, with almost 100% sequence identity. However, proteomic analysis of zdt-9n2 yielded no proteins with the potential to conferantibiotic resistance. Thus, the two clones, NR-YP1 and NR-YP3, appear to be nearly 100% identical in their cloned DNA but yet express two different antibiotic resistance phenotypes.Genetic loci responsible for the resistance could not be identified in this study. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/3304 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Metagenomics | en_US |
| dc.subject | Antibiotic resistance | en_US |
| dc.subject | Spectinomycin | en_US |
| dc.subject | Nalidixic acid | en_US |
| dc.title | Detection of Antibiotic Resistance in the Environment using Functional Metagenomics | en_US |
| dc.type | Thesis | en_US |