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The interaction of stimulated mouse macrophages with vaccinia virus in vitro.

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dc.contributor.author Ward, John E.
dc.date.accessioned 2012-12-11T17:56:44Z
dc.date.available 2012-12-11T17:56:44Z
dc.date.created 1980 en_US
dc.date.issued 2012-12-11
dc.identifier.uri http://hdl.handle.net/123456789/2265
dc.description viii, 53 leaves en_US
dc.description.abstract Stimulated mouse peritoneal macrophagcs were studied to determine the mechanism by which they express resistance to vaccinia virus. Macrophages from non-immunized animals were harvested and infected with vaccinia in vitro. The virus failed to replicate within the macrophage culture. Autoradiographic studies revealed that no significant viral DNA synthesis occurred in infected macrophages. An uncoating assay yielded evidence that vaccinia did not undergo replicative second stage uncoating within the mouse macrophage. Examination of infected macrophaees with the electron microscope indicated that vaccinia was retained within the phagocytic vacuole of the macrophage where it underwent at least partial degradation. Because no portion of the viral replicative sequence was detected, the stimulated mouse macrophange appears to be a resistant rather than a non-permissive cell type for vaccinia. It is suggested that the level of mouse macrophage activation may be an important factor in vaccinia resistance. en_US
dc.language.iso en_US en_US
dc.subject Macrophages-Research. en_US
dc.subject Vaccinia-Research. en_US
dc.title The interaction of stimulated mouse macrophages with vaccinia virus in vitro. en_US
dc.type Thesis en_US
dc.college las en_US
dc.advisor Helen McElree en_US
dc.department biological sciences en_US

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