Emporia ESIRC

LDH activity and isozyme patterns in crayfish (Orconectes nais) under conditions of seasonal change.

ESIRC/Manakin Repository

Show simple item record

dc.contributor.author Chen, Li-Hua.
dc.date.accessioned 2012-06-21T18:39:25Z
dc.date.available 2012-06-21T18:39:25Z
dc.date.created 1997 en_US
dc.date.issued 2012-06-21
dc.identifier.uri http://hdl.handle.net/123456789/1451
dc.description ix, 48 leaves en_US
dc.description.abstract In the crayfish Orconectes nais, LDH is present in two isozymatic forms. Isozymes from gill, abdominal muscle, heart and nerve have the same mobility indicating that they possess the same polypeptide composition. However, isozymes from hepatopancreas and intestine moving more cathodically suggests these isozymes are more positively charged. Isozyme patterns did not differ under different seasons suggesting no seasonal changes in the LDH isozyme composition of each tissue. The relative activities of the isozymes demonstrate that LDHs from gill, abdominal muscle, heart, and nerve are all M-type. These M-type LDHs with a ratio (0.33 mM pyruvate substrate/10 mM pyruvate substrate) close to 0.5 show that activity increases with an increase in pyruvate concentration. This is different from that of rat skeletal muscle which has a ratio of 1.67 indicating isozyme inhibition with high pyruvate concentrations. The effect of protease inhibitors on LDH activities from hepatopancreas and intestine suggests that hepatopancreas has relatively low LDH activity. Gill, abdominal muscle, heart, -and nerve show seasonality in the level of LDH activity. The physiological significance of the seasonality in LDH activity for seasonal adaptation is discussed. Key words: Lactate dehydrogenase; LDH; Orconectes nais. en_US
dc.language.iso en_US en_US
dc.subject Crayfish. en_US
dc.subject Lactate dehydrogenase. en_US
dc.title LDH activity and isozyme patterns in crayfish (Orconectes nais) under conditions of seasonal change. en_US
dc.type Thesis en_US
dc.college las en_US
dc.advisor Gaylen J. Neufeld en_US
dc.department biological sciences en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record