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Genetic variability of Escherichia coli 0157:H7.

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dc.contributor.author Tseng, Ching Wen.
dc.date.accessioned 2012-06-05T15:02:31Z
dc.date.available 2012-06-05T15:02:31Z
dc.date.created 1999 en_US
dc.date.issued 2012-06-05
dc.identifier.uri http://hdl.handle.net/123456789/1205
dc.description ix, 61 leaves en_US
dc.description.abstract Escherichia coli 0157:H7 is recognized as an important food-borne pathogen, being implicated in over 2,500 deaths worldwide. Various genetic typing methods have been investigated in studying possible outbreak strains, including pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPO), and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) . These genetic methods yield information on the genetic diversity of organisms, but make no attempt to identify specific virulence genes. In this study, we present a modified RAPO which targets specific virulence genes under low stringency conditions and also yields information on the diversity of the organism in question. Plasmid profiling, ERIC-I peR, and RAPO were also used to determine genetic heterogeneity for comparison. Our results indicate the modified RAPO may be a better tool for investigating the genetic relationships of organisms. en_US
dc.language.iso en_US en_US
dc.subject Escherichia coli. en_US
dc.title Genetic variability of Escherichia coli 0157:H7. en_US
dc.type Thesis en_US
dc.college las en_US
dc.advisor Scott Crupper en_US
dc.department biological sciences en_US

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