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  • Torres, Emily; Simons, Kim (2011-10-20)
    Two methods were employed to achieve soluble expression of Cdc14p. The first being the retrieval of CDC14 from a pET28 expression plasmid and insertion into a GST plasmid by use of the restriction enzyme NdeI. Before this ...
  • Kaiser, Chelsi; Simons, Kim (2011-10-20)
    The end of mitosis is marked by a duplication of cellular DNA, segregation of chromosomes to opposite sides of the mitotic spindle, and proper alignment of the spindle within the dividing cell. If any of these events are ...